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The last proposed system of RBC vasodilation in the presence of nitrite is the reduction of nitrite to NO by Hb. Brooks initially studied the nitrite Hb interaction in 1937 which do the job was later on prolonged by Doyle in 1981 (Brooks, 1937; Doyle et al., 1981). Nitrite reacts with deoxygenated Hb to form metHb and NO (Brooks, 1937; Doyle et al.